Expression analysis of putative small regulatory RNAs in Mycobacterium tuberculosis: effects of
growth phase and oxidative stress
MTK Au, EWC Chan, RCY Chan
Department of Microbiology, The Chinese University of Hong Kong
Key Message
Results from northern blotting and RT-PCR can
reveal which putative genes are authentic small RNA-coding
elements and provide preliminary data on the
induction characteristics of these important genetic
regulators, thereby extending the number of small
RNAs in MTB that are experimentally detectable at
a transcriptional level, and fostering future research
to delineate their regulatory role in this pathogen.