The prone table machine is a mammographic X-ray system specially designed for use in the stereotactic localisation of breast abnormality. In this study, its clinical usefulness was investigated in terms of duration, success rate, complications, and patients’ acceptance of the procedure. During a 5-month period, 79 patients attended the Kwong Wah Hospital for stereotactic-guided biopsy on the prone table. Eighty-one lesions were assessed—seven by fine needle aspirations, 67 by large-core needle biopsies, and seven by vacuum-assisted biopsies. Most of the biopsies were done because of clustered microcalcifications (77.8%) and the majority were of mammographically indeterminate nature (58.0%). The mean duration of the procedure was 49 minutes. A high degree of acceptance was experienced by patients. Only one patient had persistent haemorrhage after the biopsy. In conclusion, the prone table machine was considered to be useful and efficient, and had a high degree of acceptance among patients.