Hong Kong Med J 2025;31(Suppl 1):S38-40
Identification of new allergens in Periplaneta americana by omics and immunological techniques for cockroach-related allergic diseases: abridged secondary publication
WNS Au1, TF Leung2, KWS Tsui3
1 School of Life Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China
2 Department of Paediatrics, The Chinese University of Hong Kong, Hong Kong SAR, China
3 School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China
 
 
  1. A high-quality, chromosome-level genome of Periplaneta americana was assembled and well-annotated, with a size of 3.06 Gb and 29 939 predicted protein-coding genes.
  2. In silico identification of putative allergens was performed in the high-quality P americana genome, revealing a complete allergen profile that includes 43 allergen groups.
  3. Proteomic analysis using immunoassay and mass spectrometry methods detected seven novel allergen groups: Per a 14, Per a 15, Per a 16, Per a 17, Per a 18, Per a 19, and Per a 20.
  4. A new isoallergen of tropomyosin (Per a 7.02) and multiple potential isoallergens of Per a 5 were identified through bioinformatics and proteomic techniques.