Hong Kong Med J 2012;18(Suppl 2):S17-21
Human immunogenic T cell epitopes in nucleoprotein of human influenza A (H5N1) virus
YK Cheung, SCS Cheng, Y Ke, Y Xie
Department of Biology, The Hong Kong University of Science and Technology, Hong Kong
1. Two novel HLA-A2.1 specific H5N1 nucleoprotein epitopes (NP373-381 AMDSNTLEL and NP458-466 FQGRGVFEL) capable of activating cytotoxic T-cells in vitro were identified.
2. When the H5N1 nucleoprotein epitopes (NP373-381 AMDSNTLEL and NP458-466 FQGRGVFEL) were used with the single chain trimer system, they elicited effective cytotoxic T-cell responses against the corresponding nucleoprotein peptide-loaded cells in an HHD transgenic mouse model.
2. When the H5N1 nucleoprotein epitopes (NP373-381 AMDSNTLEL and NP458-466 FQGRGVFEL) were used with the single chain trimer system, they elicited effective cytotoxic T-cell responses against the corresponding nucleoprotein peptide-loaded cells in an HHD transgenic mouse model.