Hong Kong Med J 2008;14(Suppl 5):S4-7
A high-throughput detection method for hepatitis B virus mutations in the Chinese population
CC Lau, WY Ha, PYK Yue, SH Chui, AKK Chui, RNS Wong
Department of Biology, Hong Kong Baptist University, Kowloon, Hong Kong
1. Arrayed primer extension (APEX) has been successfully developed as a high-throughput method for single nucleotide polymorphisms (SNPs) present in the hepatitis B virus (HBV) genome.
2. Co-existence of wild-type and mutant genotypes in one SNP was successfully identified by APEX, which was not identified by conventional DNA sequencing.
3. Thirty-three patients were involved in this study; APEX results showed A1762T and G1764A double mutations and G1896A mutation are the most prevalent SNPs present in HBV genomes.
4. APEX can be developed as a SNP screening tool to monitor other infectious agents.
2. Co-existence of wild-type and mutant genotypes in one SNP was successfully identified by APEX, which was not identified by conventional DNA sequencing.
3. Thirty-three patients were involved in this study; APEX results showed A1762T and G1764A double mutations and G1896A mutation are the most prevalent SNPs present in HBV genomes.
4. APEX can be developed as a SNP screening tool to monitor other infectious agents.